Aim:

To find and extract data from original studies that assess the detection of transgenic DNA or newly expressed proteins in livestock animals fed with GMO-containing feeds.

Selection criteria:

In order to be included in the database a study needs to include each of the following criteria:

Relevant population(s): Cattle, pig, sheep, goat, poultry and fish.

Relevant exposure: Feed containing genetically modified organisms (GMO).

Relevant comparator: Feed containing the non-genetically modified conventional counterpart of the respective GMOs

Relevant outcomes: Any measurements of the presence or absence of transgenic DNA or newly expressed proteins in animal tissues, organs or fluids.

Search strategy:

The search of original research articles was performed using Scopus ( http://www.scopus.com ), a large abstract and citation database of peer-reviewed literature using the following search string: TITLE-ABS-KEY ( "genetically modified"  OR  "genetically engineered"  OR  biotech*  OR  transgenic )  AND  TITLE-ABS-KEY ( cattle  OR  dairy  OR  cow*  OR  goat  OR  rabbit  OR  farm*  OR  fish  OR  poultry  OR  chicken  OR  hen  OR  pig*  OR  sheep  OR  calf )  AND  TITLE-ABS-KEY ( fed  OR  feeding  OR  consumption  OR  diet )  AND  TITLE-ABS-KEY ( maize  OR  corn  OR  rice  OR  soybean  OR  soya  OR  rapeseed  OR  potato )

Appling selection criteria:

One reviewer performed the selection of the studies, which fulfil the inclusion criteria at three stages. At first, articles are selected by their title to remove highly irrelevant studies from the overall search results, followed by the second stage in which the inclusion criteria are applied against the abstracts of the articles. In the third stage, the articles are reviewed in full text for the presence of each of the elements needed for the inclusion.

Data extraction:

Details about the performed experiment were extracted from the "Materials and Methods" section of the papers, while the measurements of the presence or absence of transgenic DNA or newly expressed proteins were extracted from the tables and graphs as provided by the authors in standardized data extraction sheet. Check for errors were performed on 20% subset of randomly selected records.

The following data extraction sheet was used:

Attribute

Definition

Type

Animal

Name of the animal

Characters

Animal_breed

Name of the animal breed

Characters

Gender

Name of the gender of the animal

Characters

Group_number

Number of animal in the group for each treatment

Number

GM_crop

Name of the crop

Characters

GM_trait

Name of the GM trait

Characters

Inserted_gene

Name of the inserted gene

Characters

GM_event

Name of the GM event

Characters

Feed_type

Name of the type of feed

Characters

GM_content_in_the_feed

Percentage of GM material in the feed

Number and characters

Duration

Period in which animals have been fed with GM feed

Number and characters

Time_point_of_sampling

Time point of the sampling from the beginning of the experiment

Number and characters

Detection_method

Name of the method used for detection

Characters

Measurement_endpoint

Name of measured endpoint such as DNA or protein

Characters

Probes_for_detection

Name of probes used for detection

Characters

Type_of_sample

Name of the type of the sample such as blood digesta content, egg excreta, milk, muscule tissue

Characters

Sample

Name of the sample

Characters

Measurement_units

Name of experiment outcome measurement

Characters

GM_treatment

Measured outcome for the GM treatment

Characters

NonGM_treatment

Measured outcome for the non-GM treatment

Characters

References

Any information which may be relevant

Characters

Full_references

Bibliographic reference

Characters

Source

Table or figure from which the data originates

Characters

Link_to_publication

Web link or DOI to the publication

Characters

 

 

           

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